Lower affinity CAR-T cells exhibit more robust antitumor activity
The initiation of this clinical trial is a significant milestone for CAR-T therapy as it utilizes CAR-T cells having roughly 1000-fold lower affinity to their target than most CAR-T cells used to date. These lower affinity CAR-T cells produce more robust and long-lasting anti-tumor activity than do high affinity CAR-T cells in animal models. Importantly, toxicity to healthy cells expressing basal levels of the target ICAM-1, seen with high-affinity CAR-T cells, was averted using CAR-T cells tuned to a lower-affinity.
AffyImmune co-founder and inventor of the technology, Moonsoo Jin, PhD stated, "this strategy can target antigens overexpressed in tumors without harming normal cells. As there are very few antigens that are purely tumor-associated, this opens the door to effectively targeting many tumor antigens that are otherwise very challenging." Regarding the low efficacy observed to date using CAR-T cells against solid tumors in other studies, he continued, "CAR-T cells with an affinity-tuned targeting system demonstrate more robust and long-lasting anti-tumor activity, suggesting that a lower affinity targeting system prevents exhaustion of T cells and may also provide better serial killing of tumor cells after CAR-T therapy."
AffyImmune CAR-T cells are traceable in vivo
In addition to enhanced longevity and anti-tumor activity, AffyImmune CAR-T cells are engineered for in vivo tracking using PET/CT imaging. Inclusion of the Somatostatin Type II receptor [SSTR2] enables real-time imaging of CAR-T cells using the approved radiotracer 68Ga-DOTATATE with PET/CT scanning. SSTR2 specifically binds to the radiolabeled somatostatin analogue 68Ga-DOTATATE.
Assessing the toxicity and efficacy of affinity-tuned CAR-T cells
Prior studies by Dr. Jin on LFA-1, a natural receptor for ICAM-1, resulted in the production of a range of LFA-1 affinity variants with a million-fold difference in binding affinities to ICAM-1. These were subsequently engineered into CAR-T cells and tested against a series of normal and tumor cells. Because LFA-1 interacts with ICAM-1 in human and murine models, toxicity and anti-tumor activity could be assessed simultaneously in animal models. Furthermore, ICAM-1 expression is upregulated by cytokines released during CAR-T cell-mediated killing of target cells. This means that if only 60% of tumor cells have high ICAM-1 levels, cytokines released during the killing of those cells cause the remaining ICAM-1-low tumor cells to upregulate ICAM-1, thereby becoming better targets themselves.
"Our preclinical data has shown that affinity-tuned CAR-T cells dramatically improve both their activity against solid tumors and safety," said Dr. Eric von Hofe, President of AffyImmune. "The green light by the FDA gives us the opportunity to demonstrate the efficacy of this approach in patients who have an otherwise incurable disease."
"We are very proud to have incubated AffyImmune since its inception," said Simone Song, Senior Partner of ORI Healthcare Fund. "The goal of AffyImmune is to use CAR-T therapy to tackle solid tumors. What AffyImmune has achieved today is based on extensive basic science research as well as the founders' perseverance at the discovery and enabling stage. Together with the management team, we hope to advance AffyImmune's program to the next stage and do our part in addressing these unmet medical needs."
About AffyImmune Therapeutics, Inc.
AffyImmune is realizing the potential of cancer immunotherapy by extending the anti-cancer activity of CAR-T cell therapy to solid tumors, improving the safety and efficacy of CAR-T treatment. Based on Professor Moonsoo Jin's work at Weill Cornell Medicine, AffyImmune's proprietary technology allows fine-tuning of the affinity of CAR-T cells to selectively kill tumor cells, while sparing healthy cells to reduce toxicity. AffyImmune scientists have also developed a tracking system that can be engineered into any CAR-T cell to allow for real-time localization of cells after administration to patients.
AffyImmune's lead asset, AIC100, is an affinity-tuned, 3rd generation CAR-T cell therapy co-expressing SSTR2. Preclinical studies in various solid tumor models demonstrated AIC100's robust and enduring tumor eradication and superior safety profile compared to standard, high-affinity CAR-T cell therapy. In 2019, AffyImmune received Orphan Drug Designation by the FDA for AIC100 and began recruitment for a Phase 1 trial to treat advanced thyroid cancer.
Founded in 2016, AffyImmune is headquartered in Natick, MA and is incubated by ORI Capital.
About ORI Healthcare Fund L.P.
ORI Healthcare Fund is a $200 million venture capital fund focused on investing in innovative companies with disruptive technologies in the healthcare industry globally. The General Partner of the Fund was founded by Ms. Simone (Hong Fang) Song, the former head of China Healthcare Investment Banking at Goldman Sachs.
Eric von Hofe
AffyImmune Therapeutics, Inc.
12 Michigan Drive
Natick, MA 01760
+1 (617) 699-1965
SOURCE AffyImmune Therapeutics, Inc.
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